Effect of Potenmic Supplementation on Immunity, Blood Metabolites, and Antioxidants Status In Broiler Chicks Fed A Low Crude Protein-Metabolizable Energy Diet

Abstract

This study evaluated the effects of Potenmic supplementation on immunity, blood metabolites, and antioxidant status in broiler chicks fed a low crude protein (CP) and metabolizable energy (ME) diet (19% CP, 2900 kcal/kg ME). A positive control (PC) diet (22% CP, 3000 kcal/kg ME) and a negative control (NC) diet were compared with NC supplemented with Potenmic at 0.20, 0.33, and 0.67 mL/L of drinking water during starter and finisher phases. The NC diet reduced antibody titers against Newcastle disease (ND), while Potenmic at 0.33 mL/L significantly improved ND immunity in both phases. For infectious bronchitis (IB), 0.20 mL/L was sufficient to enhance antibody response. The heterophil-to-lymphocyte (H:L) ratio, a stress indicator, decreased with Potenmic supplementation, showing a significant reduction of 0.18 points at 0.33 mL/L in the finisher phase. Blood glucose levels increased by 43.70 mg/dL at the highest Potenmic dose (0.67 mL/L) but stabilized at lower concentrations. Serum protein levels improved across all Potenmic doses. Antioxidant status, assessed through malondialdehyde (MDA) levels, indicated reduced oxidative stress at 0.67 mL/L (starter) and 0.33 mL/L (finisher). In conclusion, Potenmic supplementation at 0.67 mL/L (starter) and 0.33 mL/L (finisher) in low CP-ME diets enhances immune response, optimizes blood metabolites, and improves antioxidant status in broiler chicks.